Screening of Cyanide-Utilizing Bacteria from Rumen and In Vitro Evaluation of Fresh Cassava Root Utilization with Pellet Containing High Sulfur Diet

Two experiments were undertaken to screen for ruminal cyanide-utilizing bacteria (Experiment 1), and evaluate the influence of fresh cassava root (FCR) pellets containing high sulfur (PELFUR) on cyanide content, gas production parameters, in vitro degradability, fermentation 2). Experiment 1 was conducted a completely randomized design (CRD) screening dietary treatments consisted at 0, 150, 300, 450 ppm. In 2, 5 × 3 factorial arrangement used study. Factor A level FCR 260, 350, 440, 530 g/kg dry matter (DM) substrate, factor B PELFUR 15, 30 DM substrate. 1, adding different doses significantly affected rumen bacterial growth (p < 0.05). Increasing concentration from 0 150 300 ppm resulted increases 38.2% 15.0%, respectively. no interaction effects found between parameters > more than 260 substrate could increase cumulative 15 increased when compared with that g PELFUR/kg The fluid decreased 0.05) supplementation. Degradability organic following incubation 12 24 h due supplementation additionally 440 FCR/kg Proportions total volatile fatty acids, acetic acid (C2), propionic (C3), butyric among supplementations different. conclusion, present results represent first finding are capable utilizing cyanide, suggests might function as nitrogen source cell synthesis. inclusion production, population, proportion C3, rate disappearance cyanide.